PEPSIN DIGESTION OF RABBIT AND SHEEP ANTIBODIES
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چکیده
منابع مشابه
Pepsin Digestion of Rabbit and Sheep Antibodies
7S sheep antibody is similar to 7S rabbit antibody, insofar as it too can be digested with pepsin to yield a 5S fragment still capable of precipitating homologous antigen. The 5S fragment from rabbit as well as sheep antibody is capable of fixing guinea pig complement. However, this fixation differs from the fixation by 7S antibody, since preformed antibody-antigen aggregates containing either ...
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The functional structure of gamma globulins and antibodies has recently come under intensive investigation through the use of reduction and alkylation of disulfide bonds (1). This method has revealed the great similarity in fundamental polypeptide chain structure among many species as well as between the different classes of immunoglobulins (2). By contrast, reduction of disulfide bonds affects...
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One of the most striking peculiarities of enzyme action is the fact that the activity of the enzyme is limited to a definite range of acidity. If the solution is more or less acid than this the enzyme is practically inactive. SSrensen 1 showed that for a number of enzymes the determiuing factor was the hydrogen ion concentration and not the total acidity of the solution. In attempting to accoun...
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Limited pepsin digestion of human plasma albumin at pH 3.5 and 0 degrees in the presence of octanoate caused cleavage at residue 307 of the albumin molecule to yield two fragments. Thw two fragments corresponding to the NH2- and the COOH-terminal halves of the molecule were isolated in yields of about 15%. The COOH-terminal fragment is a mixture in which about 85% of the molecules had an additi...
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A pepsin antibody fragmentation procedure was trialed and optimised. The procedure used was a modified version of that described in Current Protocols in Immunology. Pilot digestions using a range of pepsin concentrations and incubation times were completed at pH 4.0 and pH 4.5. The digestion products were examined by Phastgel electrophoresis, and the combination of pepsin concentration, incubat...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1963
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.118.6.891